مطالعه خواص ضد‌میکروبی پوشش‌ نانوکامپوزیتی حاوی نانوذرات نقره

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری بهداشت مواد غذایی، واحد علوم و تحقیقات،دانشگاه آزاد اسلامی، تهران، ایران

2 دانشیار گروه علوم و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار گروه تخصصی علوم پایه و بهداشت، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

4 استاد گروه بهداشت مواد غذایی، دانشگاه تهران، تهران، ایران

چکیده

امروزه ذرات فلزی به پلیمرهای مورداستفاده در بسته‌بندی مواد غذایی اضافه می‌شوند تا خواص مکانیکی و ضدمیکروبی را بهبود دهند. در این مطالعه خواص ضدمیکروبی پوشش‌های نانو کامپوزیت‌ با دانسیته کم (LDPE) حاوی 5/17، 5/12، 5/7 و 5/2 درصد نانوذرات نقره بررسی گردید. برای تولید نانوذرات نقره روش احیاء شیمیایی با استفاده از احیاکننده ‌تری‌سدیم سیترات انتخاب شد. به‌منظور تأیید سنتز نانو ذرات نقره، مشخصه‌یابی توزیع ذرات نقره و ساختار کریستالی نقره به‌ترتیب از آزمون‌های UV-Visible، SEM و XRDاستفاده شد. نانو ذرات بلورین با متوسط اندازه 20 نانومتر تأیید شدند. به‌منظور بررسی خاصیت ضدمیکروبی پوشش‌ها، رشد باکتری‌ در حضور پوشش‌های نانو نقره با درصدهای مذکور به روش سنجش دانسیته نوری (OD) به‌وسیله دستگاه اسپکتروفتومتر در طول‌موج 600 نانومتر انجام گرفت. میزان مهاجرت ذرات نانو نقره نیز اندازه‌گیری شد. نتایج نشان داد که پوشش حاوی 5/17درصد نانو نقره بیشترین تأثیر را داشت و پوشش حاوی 5/2 درصد فاقد هرگونه تأثیر بود. همچنین مقایسه تأثیر پوشش‌ها بر اشریشیا کولای و استافیلوکوکوس اورئوس نشان داد که تأثیر پوشش‌ها روی استافیلوکوکوس اورئوس بیشتر بوده است. نتایج حاکی از وابستگی بین درصد نانوذرات نقره استفاده‌شده و خاصیت ضدمیکروبی بود. به‌این‌ترتیب که با افزایش درصد نانوذرات نقره، خاصیت ضدمیکروبی افزایش پیدا کرد. همچنین با افزایش درصد نانوذرات نقره میزان رهایش نانوذرات از پوشش نیز افزایش یافت. درنهایت  با توجه به‌قرار گرفتن میزان مهاجرت نانو ذرات نقره از هر چهار نوع پوشش نانوکامپوزیتی در محدوده مجاز، پوشش 5/17 درصد به‌عنوان کارآمدترین پوشش انتخاب شد.

کلیدواژه‌ها


عنوان مقاله [English]

Antimicrobial properties of nanocomposite films containing silver nanoparticles

نویسندگان [English]

  • M. Pouyamanesh 1
  • H. Ahari 2
  • A.A. Anvar 3
  • G. Karim 4
1 Ph.D candidate in Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Associate Professor, Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Assistant Professor, Department of Basic Sciences and Health, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 Professor, Department of Food Hygiene, Tehran University, Tehran, Iran
چکیده [English]

Metal particles are added to polymers used in the food packaging to improve their mechanical and antimicrobial properties. In this study, the antimicrobial properties of low-density polyethylene nanocomposite (LDPE) containing 17.5%, 12.5%, 7.5%, and 2.5% silver nanoparticles were investigated. UV-Visible, SEM, and XRD tests were used to confirmation of the synthesis of silver nanoparticles, characterization of silver particle distribution, and silver crystal structure. Crystal nanoparticles with an average size of 20 nanometers were approved. To evaluate the antimicrobial properties of silver nanocomposites, bacterial growth in the presence of nanosilver films with the mentioned percentages was measured by optical density (OD) method via spectrophotometer (600 nm). Besides, the migration of nanoparticles was measured. The results showed that the coating containing 17.5% and 2.5% nanosilver had the highest and the lowest effect, respectively and in comparison to the other groups. Also, comparing the effect of coatings on two types of food pathogens, E. coli and S. aureus, showed that the effect of coatings on S. aureus was higher. The results showed a correlation between the percentage of silver nanoparticles used and antimicrobial properties. Furthermore, with increasing the percentage of silver nanoparticles, the releasing rate of nanoparticles from the coating increased. Finally, due to the migration of silver nanoparticles from all four types of nanocomposite coatings in the permitted range, 17.5% coating was selected as the most efficient one.

کلیدواژه‌ها [English]

  • Silver Nanocomposite
  • Antibacterial
  • Migration
 

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