اثر ضدباکتریایی نانوذره آلومینات روی بر باکتری‌های بیماری‌زای‌ غذایی اشریشیا کولای و سودوموناس آئروژینوزا

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

2 استادیار شیمی آلی، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

3 دانشجوی کارشناسی ارشد فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

چکیده

مواد در ابعاد نانو که از نسبت سطح به حجم بالایی برخوردارند واکنش‌پذیری بهتری با سایر ترکیبات دارند. گسترش فناوری نانو در دهه‌ی اخیر، فرصت‌هایی برای کشف اثرات ضد باکتریایی نانو ذرات ایجاد کرده است. در این پژوهش خواص ضد باکتریایی نانوذره روی آلومینات(ZnAl2O4) علیه باکتری‌های عامل بیماری‌های غذازاد  اشریشیا کولای و سودوموناسآئروژینوزا بررسی شد. با استفاده از نانوذره روی آلومینات سنتز شده فعالیت ضد باکتری به روش انتشار دیسک بر آگار مورد ارزیابی قرار گرفت. قطر هاله‌های عدم رشد نشان‌دهنده میزان حساسیت باکتری‌ها به نانو مواد مورداستفاده بود. حداقل غلظت مهارکنندگی از رشد باکتری‌ها (MIC) و حداقل غلظت کشندگی باکتری‌ها (MBC) به روش ماکرودایلوشن در لوله‌های استریل آزمایشگاهی انجام گرفت. بررسی آماری با آزمون T test انجام گرفت. با استفاده از نتایج به‌دست‌آمده در این بررسی قطر هاله عدم رشد سودوموناس آئروژینوزا (8/1 ± 06/16 میلی­متر) نسبت به اشریشیاکولای (2/2±64/11 میلی­متر) بیشتر بود (p < 0.05). کمترین غلظت مهارکننده رشد و غلظت کشندگی باکتری‌ها مربوط به باکتریسودوموناس آئروژینوزا بود و باکتری اشرشیاکولای فاقد آن بود. با توجه به نتایج به‌دست‌آمده در این مطالعه نانو ذرات روی آلومینات علیه باکتریسودوموناس آئروژینوزا فعالیت ضد باکتری بسیار خوبی نشان داد و می‌تواند به‌عنوان یک ترکیب ضدباکتریایی جدید در مطالعات بسته‌بندی مواد غذایی مورداستفاده قرار گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

Antibacterial activity of zinc aluminate nanoparticles against foodborne pathogenic bacteria of E. coli and P. aeruginosa

نویسندگان [English]

  • A. Taheri 1
  • M. Ziaadini 2
  • M. Gahramzei 3
1 Associate Professor, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
2 Assistant Professor, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
3 Graduate Student, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran
چکیده [English]

Materials in nanoscale dimensions have a high surface-to-volume ratio, and thus have better reactions with other materials. The development of nanotechnology over the past decades has opened up opportunities for discovering the antibacterial effects of metallic nanoparticles. In this study, the antibacterial properties of nanoparticles on aluminate (ZnAl2O4) were studied on Escherichia coli and Pseudomonas aeruginosa bacteria. Antibacterial activity was assessed by using the disk diffusion method on agar using nanoparticles on synthesized aluminate. The non-growth halo diameter indicates the sensitivity of bacteria to antimicrobials. The minimum inhibitory concentration of bacterial growth (MIC) and minimum bacterial leaching concentration (MBC) was measured by microdilution in sterile tubes. Statistical analysis was done by T-test. The results of this study showed that Pseudomonas aeruginosa sensitivity (16.06±1.8mm) was higher than E. coli (11.64±2.2mm) (p < 0.05). The minimum inhibitory concentration (MIC) and bactericidal concentration MBC of bacteria Pseudomonas aeruginosa and Escherichia coli bacteria MIC and MBC was lacking. According to the results obtained in this study, we conclude that Pseudomonas aeruginosa was more sensitive to alumina than that of E. coli against nanoparticles.

کلیدواژه‌ها [English]

  • Antibacterial activity
  • Zinc aluminate
  • Nanoparticle
  • Escherichia coli
  • Pseudomonas aeruginosa
 

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