ارزیابی خصوصیات پروبیوتیکی و ضدقارچی باکتری اسیدلاکتیک غالب جداشده از خمیرترش جو دوسر

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد زیست‌فناوری مواد غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 دانشیار، گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان

3 استاد گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

4 استادیار گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

چکیده

ارزیابی ویژگی­های پروبیوتیکی و ضدقارچی باکتری­های اسیدلاکتیک جداشده از انواع خمیرترش به‌منظور تأمین کشت­های میکروبی مورداستفاده در صنایع تخمیری از اهمیت بسزایی برخوردار است. در این پژوهش، باکتری اسیدلاکتیک غالب با تکرار فرایند مایه­گیری از خمیرترش جو دوسر، جداسازی و با استفاده از (Polymerase chain reaction) PCR شناسایی گردید. سپس ویژگی­های پروبیوتیکی و همچنین اثر ضدقارچی این جدایه لاکتیکی روی آسپرژیلوس نایجر (Aspergillus niger) مورد مطالعه قرار گرفت. توالی­یابی محصولات PCR منجر به شناسایی پدیوکوکوس پنتازاسئوس (Pediococcus pentosaceus) به­عنوان جدایه لاکتیکی غالب خمیرترش جو دوسر شد. نرخ زندهمانی جدایه مذکور پس از تیمار متوالی اسید و صفرا در مقایسه با نمونه شاهد 80/59 درصد بود. همچنین اثر ضدباکتریایی آن در برابر باسیلوس سرئوس (Bacillus cereus) نسبت به سایر عوامل بیماری­زای موردمطالعه به شکل معنی­داری (p<0.05) بیشتر بود. روماند خام فاقد سلول (Crude cell-free supernatant) جدایه لاکتیکی نیز به­طور کامل از رشد باسیلوس سرئوس ممانعت نمود اما رشد سالمونلا انتریکا (Salmonella enterica) را 65/68 درصد کاهش داد. بااین‌حال، روماند فاقد سلول (Cell-free supernatant) خنثی‌شده جدایه لاکتیکی، اثر بازدارنده­ای روی این باکتری­ها نداشت. علاوه بر این، جدایه لاکتیکی از قابلیت دگر اتصالی (Co-aggregation) مناسبی با اشریشیا کولای (Escherichia coli) برخوردار بود و به آنتی­بیوتیک­های استرپتومایسین، سیپروفلوکساسین، نالیدیکسیک اسید و ونکومایسین، مقاومت نشان داد. این جدایه لاکتیکی، فاقد فعالیت همولیتیکی بود و اثر ضدقارچی آن در برابر آسپرژیلوس نایجر نیز مورد تأیید قرار گرفت. بر این اساس، جدایه پدیوکوکوس پنتازاسئوس از قابلیت مناسبی برای استفاده به­عنوان کشت پروبیوتیک و یا نگه‌دارنده در صنایع تخمیری برخوردار می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of probiotic and antifungal properties of the predominant LAB isolated from oat sourdough

نویسندگان [English]

  • Fahimeh Hajinia 1
  • A. Sadeghi 2
  • A. Sadeghi Mahoonak 3
  • M. Khomeiri 3
  • Y. Maghsoudlou 3
  • A. Moayedi 4
1 M.Sc. student of Food Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Associate professor, Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources
3 professor, Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gonrgan, Iran
4 Assistant professor, Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gonrgan, Iran
چکیده [English]

Evaluation of probiotic and antifungal properties of the lactic acid bacteria (LAB) isolated from different sourdoughs is so important to prepare microbial cultures for fermentation industries. In the present study, a predominant LAB was isolated from oat sourdough through the back-slopping process, and then it was identified using PCR. Subsequently, probiotic properties of the LAB isolate (including resistance to acid and bile, antibacterial effect, auto and co-aggregations capabilities, antibiotic susceptibility and hemolytic activity), as well as its antifungal activity against Aspergillus niger were studied. Sequencing results of the PCR products led to the identification of Pediococcus pentosaceus as predominant LAB isolated from oat sourdough. The survival rate of the LAB isolates after continuous acid and bile treatment was 59.80% in comparison with the control. The antibacterial effect of the LAB on Bacillus cereus was also significantly (p<0.05) higher than the other studied food-borne pathogenic agents. Crude cell-free supernatant (CFS) of the LAB completely inhibited the growth of B. cereus, but reduced the growth of Salmonella enterica by 65.68%. Meanwhile, naturalized CFS of the LAB had no inhibitory effect on these bacteria. Furthermore, the LAB isolates had a proper co-aggregation with Escherichia coli, and showed resistance towards streptomycin, ciprofloxacin, nalidixic acid and vancomycin antibiotics. The LAB isolates had no hemolytic activity, and its antifungal effect on A. niger was also approved. Accordingly, P. pentosaceus isolate has proper potential to use as probiotic or preservative microbial culture in fermentation industries.

کلیدواژه‌ها [English]

  • antifungal effect
  • Predominant LAB isolates
  • Probiotic properties
  • Oat sourdough

 

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