ویژگی‌های پروبیوتیکی و فعالیت ضدمیکروبی لاکتوباسیلوس‌های جدا شده از لوله گوارش مرغ‌های بومی جنوب‌غرب و شمال‌غرب ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار منطقه شمال کشور، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO)، رشت،

2 دانشیار، بخش تحقیقات میکروبی، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO)، کرج، ایران

3 کارشناس ارشد منطقه شمال کشور، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO)،

4 کارشناس منطقه شمال کشور، پژوهشکده بیوتکنولوژی کشاورزی ایران (ABRII)، سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO)، رشت، ایران

چکیده

پروبیوتیک­ها به­عنوان استراتژی کنترل‌ زیستی پاتوژن‌های گوارشی مشترک منقله از غذا در ارتقاء امنیت غذایی مصرف کننده مورد توجه هستند. هدف از این پژوهش بررسی خصوصیات پروبیوتیکی و توانایی مهار پاتوژن‌های گوارشی منتقله از غذا توسط باکتری­های اسید لاکتیکی جدا­سازی شده از مجرای گوارش مرغ­های بومی بود. در این مطالعه توانایی تحمل اسید و صفرا در 216 جدایه اسید لاکتیکی بررسی گردید و 13 جدایه برتر به­منظور توالی­یابی ژن 16 S rRNA انتخاب شدند. سپس توانایی مهار پاتوژن‌های گوارشی مشترک بین انسان و طیور توسط جدایه­های  انتخابی بررسی گردید. بررسی ژن 16S rRNA در این مطالعه مشخص نمود که تمامی جدایه­های  متعلق به جنس­های لاکتوباسیلوس بودند. از سیزده جدایه جدا شده، 7 جدایه متعلق به گونه لاکتوباسیلوس روتری ((Lactobacillus reuteri، 3 جدایه لاکتوباسیلوس کریسپاتوس (Lactobacillus crispatus) و 3 جدایه لاکتوباسیلوس سالیواروس (Lactobacillus salivarius) بودند. تمام جدایه­­ها قادر به تحمل3= pH به‌مدت  3 ساعت و توانایی تحمل 3/0 درصد صفرا بودند. از میان جدایه­های مورد بررسی، 3 جدایه متعلق به گونه L.salivarius و یک جدایه متعلق به گونه­ L. reuteri، از توانایی بهتری جهت مهار پاتوژن‌های گوارشی مشترک بین انسان و طیور برخوردار بودند. این مطالعه مشخص نمود که پتانسیل پروبیوتیکی مناسب در شماری از جدایه­های لاکتوباسیل جداسازی شده از روده مرغان بومی ایران در شرایط برون­ تنی (in vitro)، آن‌ها را تبدیل به کاندیدهای مناسبی در ترکیب مکمل­های پروبیوتیکی جهت ارتقاء امنیت غذایی مصرف کننده پس از انجام آزمایش‌ها درون تنی (in ovo) می­نماید.

کلیدواژه‌ها

عنوان مقاله [English]

The probiotic and antagonistic properties of isolated lactobacilli from the intestine of free-range chickens from southwest and northwest of Iran

نویسندگان [English]

  • M. R. 1
  • M. Hashemi 2
  • R. Seighalani 3
  • H. Alaie Kordghashlaghi 4
1 Assistant Professor, North Region Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
2 Associate Professor, Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO),Karaj, Iran
3 M.Sc, North Region Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
4 M.Sc. Technical Support in Genomics Department, North Region Branch, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran
چکیده [English]

Probiotics as a replacement for growth promoter antibiotics and foodborne pathogens bio-control strategy are attractive in the broiler chicken industry and improve consumer food safety. This research was designed to investigate the probiotic properties of lactic acid bacteria isolated from the digestive tract of local domestic chickens in Iran. In this study, the persistence of 216 lactic acid bacteria isolates was investigated to acid and bile and 13 superior isolates were selected to be identified using the 16S rRNA gene sequencing. Afterward, the ability of 13 selected lactobacilli isolates to control foodborne pathogens was studied. The results of the 16S rRNA gene sequencing showed that all isolates belonged to the Lactobacillus species. Of the 13 isolates, 7, 3 and 3 isolates belonged to Lactobacillus reuteri, Lactobacillus crispatus and Lactobacillus salivarius,respectively. All of these isolates were able to tolerate pH= 3 for 3 hours and 0.3% bile salts. Three isolates belonging to L. salivarius were more effective to inhibit common Zoonotic bacteria. This study revealed that the in vitro favorable probiotic potential in some isolated lactobacilli from the native chicken digestive tract from Iran, introduce them as good candidates in probiotic supplements to improve food safety of consumer after conducting in vivo tests.

کلیدواژه‌ها [English]

  • Intestinal pathogens
  • Iranian Native chicken
  • Lactobacilli
  • Probiotic
  • 16 S rRNA sequencing

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