خصوصیات بیوشیمیایی و ضدمیکروبی باکتری های اندوفیتی جدا شده از بومادران (Achillea millefolium) و کتیرا (Astraglus gossypinus) مناطق جغرافیایی استان فارس

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری تخصصی بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران

2 استاد بخش بهداشت مواد غذایی دانشکده دامپزشکی، دانشگاه شیراز، شیراز، ایران

چکیده

گیاهان دارویی بومادران (Achillea millefolium) و کتیرا (Astraglus gossypinus) به‌علت خواص درمانی متعدد در منطقه خاورمیانه مورد استفاده قرار می‌گیرد. حضور میکروب‌های اندوفیت در گیاهان دارویی در سال­های اخیر مورد توجه محققین قرار گرفته است. اندوفیت­ها باکتری­ها و کپک­هایی هستند که درون بافت گیاهان حضور دارند و علاوه بر فوایدی که برای گیاه دارند حاوی ترکیبات زیست‌فعال مؤثری می­باشند. در این تحقیق باکتری­های اندوفیت از گیاهان دارویی بومادران و کتیرا جداسازی شدند. اثر ضدباکتریایی و ضدکپکی آن‌ها با روش انتشار دیسکی بر روی استافیلوکوکوس اورئوس (ATCC 6538)، اشریشیا کولای (ATTC 35218)، سالمونلا تیفی­موریوم (ATCC 14028)، لیستریا مونوسیتوجنز (PTCC 1163) و آسپرژیلوس نایجر (PTCC 5154) مورد بررسی قرار گرفت. باکتری­هایی که اثر ضدمیکروبی داشتند از طریق تعیین توالی ژن16S rDNA، خصوصیات بیوشیمیایی و آنزیمی مورد شناسایی دقیق­تر قرار گرفت. باکتری‌های جداسازی شده از گیاه بومادران و کتیرا به‌ترتیب باسیلوس سافینیس و باسیلوس پومیلوس بودند که اثر مهاری روی کپک مورد آزمایش را داشتند. باسیلوس سافینیستوانایی تخمیر قندهای گلوکز، ساکاروز، لاکتوز و مانیتول را داشت. در حالی‌که باسیلوس پومیلوس جداسازی شده از گیاه کتیرا تنها گلوکز را تخمیر کرد و دارای آنزیم­های اکسیداز، کاتالاز و آمیلاز بود.

کلیدواژه‌ها


عنوان مقاله [English]

Biochemical and antimicrobial properties of bacterial endophytes isolated from yarrow (Achillea millefolium) and tragacanth (Astragalus Gossypium) in Fars geographical regions

نویسندگان [English]

  • Z. Erjaee 1
  • S.S. Shekarforoush 2
  • S. Hosseinzadeh 2
1 Ph.D. student of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
2 Professor of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
چکیده [English]

Yarrow (Achillea millefolium) and tragacanth (Astragalus Gossypium) are medicinal plants in the Middle East that have been used for medical purposes. The presence of endophytic bacteria in the medicinal plants has been recently focused by many researchers. Endophytes are bacteria and fungi in the inner parts of the plant tissues which in addition to their benefits for the plants, contain effective bioactive compounds. In this research, endophytic bacteria were isolated from yarrow and tragacanth. Their antifungal and antibacterial activities against Staphylococcus aureus (ATCC 6538), Escherichia coli (ATTC 35218), Salmonella Typhimurium(ATCC 14028), Listeria monocytogenes (PTCC 1163) and Aspergillus niger (PTCC 5154) were assessed using disk diffusion method. Bacteria having antimicrobial activity were further identified by 16S rDNA, biochemical characteristics and enzyme activities. The bacteria isolated from yarrow and tragacanth were found as Bacillus safensis and Bacillus pumilus, respectively. These isolates showed inhibitory effects against the tested fungi. B. safensis could ferment D- glucose, sucrose, mannitol, and lactose. The activities of oxidase, catalase, gelatinase, protease, and amylase enzymes were also investigated. B. pumilus could only ferment D-glucose and represented the activities of oxidase, catalase, and amylase.

کلیدواژه‌ها [English]

  • Endophytic bacteria
  • yarrow
  • Tragacanth
  • Bacillus pumilus
  • Bacillus safensis
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