ارزیابی ویژگی‌های کشک پروبیوتیک در شرایط پر کردن داغ حاوی لاکتوباسیلوس ‌رامنوسوس ریزپوشانی شده

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دکتری تخصصی، گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه شیراز، فارس، ایران؛ مربی، عضو هیئت علمی بخش تحقیقات فنی مهندسی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی فارس، سازمان تحقیقات و آموزش جهاد کشاورزی، شیراز، ایران

2 استاد، گروه بیوشیمی و بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه شیراز، فارس، ایران

3 دانشیار، گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه شیراز، فارس، ایران

4 استاد، گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه شیراز، فارس، ایران

5 دانشیار، گروه صنایع غذایی ، دانشکده کشاورزی، دانشگاه شیراز، فارس، ایران

چکیده

کشک یکی از محصولات تخمیری پرطرفدار در ایران می‌باشد، که به‌‌صورت داغ تولید و بسته‌بندی می‌گردد. به‌علت حساس بودن باکتری‌های پروبیوتیک به حرارت، تاکنون اقبالی جهت تولید کشک پروبیوتیک صورت نگرفته است. در این تحقیق از تکنیک ریز‌پوشانی اکستروژن دولایه توسط آلژینات و صمغ زودو بر روی باکتری لاکتوباسیلوس ‌رامنوسوس انجام شد و مؤلفه‌های اسیدیته، رنگ، بافت، ویژگی‌های حسی و زنده‌مانی باکتری پروبیوتیک مورد سنجش قرار گرفت. نتایج نشان داد که اسیدیته تا روز 60 در نمونه شاهد ثابت و در حدود 211 درجه دورنیک و در نمونه پروبیوتیک در حدود 193 درجه دورنیک و از روندی ثابت برخوردار بود.­ باکتری آزاد اضافه‌ شده به نمونه کشک، بعد از پروسه پرکردن داغ، ماندگاری نداشت ولی نمونه حاوی دانک تا روز چهاردهم نگهداری، از استاندارد حد مجاز لازم برای محصول پروبیوتیک برخوردار بود. در روز شصت، مؤلفه‌های حسی، نظر کلی، طعم، قوام و دل‌پذیری در نمونه کشک شاهد دارای امتیاز بالاتری نسبت به کشک پروبیوتیکی بود. EΔ‌ در کشک شاهد 8/4 و در کشک پروبیوتیک 62/2 بود. به‌غیر از پیوستگی، سایر مؤلفه‌های بافتی در کشک شاهد و حاوی دانک در ابتدای مدت نگه‌داری افزایش یافت و در طول مدت نگه‌داری نسبتاً ثابت ماند. زنده‌مانی باکتری لاکتوباسیلوس رامنوسوس ریزپوشانی شده دولایه، در حداقل تعداد مجاز log cfu/ml 6، برای محصول پروبیوتیک، 14 روز بود و مدت زمان ماندگاری کشک شاهد 60 روز بود. لذا این تکنیک می‌تواند به‌عنوان یک روش مؤثر در ماندگاری باکتری پروبیوتیک در کشک به‌کار رود

کلیدواژه‌ها

عنوان مقاله [English]

The evaluation of probiotic Kashk properties in hot filling condition containing microencapsulated Lactobacillus rhamnosus

نویسندگان [English]

  • S. sekhavatizadeh 1
  • M. aminlari 2
  • H.R. Gheisari 3
  • S. shekarfroosh 4
  • M. Golmakani 5
1 Ph.D. graduate of Food Hygiene, School of Veterinary Medicine, Shiraz University, Fars, Iran
2 Professor, Biochemistry and Food Hygiene Department, School of Veterinary Medicine, Shiraz University, Fars, Iran
3 Associate Professor, Food Hygiene Department, School of Veterinary Medicine, Shiraz University, Fars, Iran
4 Professor, Food Hygiene Department, School of Veterinary Medicine, Shiraz University, Fars, Iran
5 Associate Professor, Food Science and Technology Department, School of Agriculture, Shiraz University, Fars, Iran
چکیده [English]

Kashk is one of the most popular fermented products in Iran which is produced and packaged in the hot filling mode. Due to the sensitivity of probiotic bacteria to heat, so far, there is no attempt to produce probiotic Kashk. Therefore, in this project, two-layered extrusion microencapsulation technique by alginate and Zedo gum was applied to Lactobacillus rhamnosus. Acidity, color, texture, sensory parameters and bacterial viability were evaluated. The results showed that the acidity was constant during the 60th day of storage time about 211‌°‌D in the control sample and‌193 °‌D in the probiotic sample. The free bacteria had not survived after the hot filling process in the Kashk sample, but the probiotic sample had a standard limit for the probiotic product until the 14th day of storage time. On the 60th day, general view, flavor, acceptance, and viscosity as sensory parameters in the control Kashk sample had a higher rating than the probiotic one. ΔE was 4.8 and 2.62 in control and probiotic Kashk, respectively. Except for cohesiveness, the other texture parameters increased at the beginning of the storage period and remained relatively constant during the time in control and probiotic Kashk samples. Therefore, according to the results of this research, the two-layered microencapsulated L. rhamonosus survived for 14 days as the recommended minimum limit of 6 log­ CFU/g bacteria for probiotic products and control Kashk had a shelf life of 60 days. Thus, this technique can be used as an effective method for the survival of probiotic bacteria in Kashk.

کلیدواژه‌ها [English]

  • Kashk
  • Lactobacillus rhamnosus
  • Microencapsulation
  • probiotics
  • Zedo

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