اثر اسانس رزماری بر جمعیت لاکتوباسیل‌ها و میزان هیستامین طی دوره رسیدن در پنیر سنتی لیقوان

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای بهداشت مواد غذائی، دانشگاه آزاد اسلامی واحد تریز، تبریز، ایران

2 دانشیار گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد تبریز، تبریز، ایران

3 استادیار گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد تبریز ، تبریز، ایران

4 استادگروه بهداشت و کنترل کیفی مواد غذائی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

5 استاد گروه بهداشت مواد غذایی، دانشکده علوم تخصصی دامپزشکی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تبریز، ایران

چکیده

مسمومیت هیستامینی یکی از نگرانی‌های مهم در سلامت پنیرهای سنتی رسیده  بوده و مهم‌ترین عامل تولید آن میکرارگانیسم‌های دکربوکسیله کننده در طی دوره رسیدن می‌باشد. هدف از مطالعه حاضرتعیین تأثیر اسانس رزماری بر جمعیت لاکتو باسیلوس‌ها و میزان هیستامین در پنیر لیقوان در طول دوره رسیدن با روش HPLC بود. ترکیب شیمیائی اسانس با استفاده از GC/MS مورد شناسائی قرار گرفت. نمونه‌های پنیر حاوی صفر، 125 و  ppm250 از اسانس رزماری تهیه شد. نمونه‌ها در روزهای صفر، 30، 90 و 150 دوره رسیدن از نظر تعداد باکترهای لاکتوباسیلوس و میزان هیستامین مورد آزمایش قرار گرفتند. آلفا‌پینن (Alfa pinene) با 1/11 درصد، کامفور ((Camphore با 2/10 درصد و لیمونن – د ( Limonene-d) با 9/5 درصد، 3 جزء عمده موجود در اسانس بود. میانگین تعداد لاکتوباسیل ها در روزهای 30، 90 و 150 دوره رسیدن در نمونه‌های حاوی 125 و ppm 250 اسانس رزماری به‌طور معنی‌داری کمتر از نمونه‌های شاهد بود (05/0>(P. در طول دوره رسیدن میزان هیستامین در نمونه‌‌های حاوی اسانس کمتر از نمونه‌های شاهد بوده و در انتهای دوره رسیدن میزان هیستامین در نمونه‌های شاهد، حاوی 125 و ppm 250 اسانس رزماری به‌ترتیب برابر 190، 170 و ppm  04/51 بود. در مجموع می‌توان گفت که غلظت‌های 125 و  ppm250 از اسانس رزماری می‌تواند به‌عنوان یک ماده نگه‌دارنده طبیعی در پنیرهای سنتی لیقوان استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Rosmarinus officinalis essential oil on lactobacillus count and amount of histamine during the ripening period in traditional Lighvan cheese

نویسندگان [English]

  • R. Shihegar 1
  • H. Mirzaei 2
  • K. mohammadi 3
  • G. Karim 4
  • V. Razavilar 5
1 Ph.D. Graduate in Food Hygiene, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Associate Professor, Department of Food Hygiene, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 Assistance Professor, Department of Food Hygiene, Tabriz Branch, Islamic Azad University, Tabriz, Iran
4 Professor. Professor, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5 Professor, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده [English]

Histamine poisoning is one of the main concerns in traditional cheeses which is mainly produced by decarboxylating bacterial strains during cheese ripening period. The aim of this study was to determine the effect of Rosmarinus officinalis essential oil (EO) on lactobacillus count and histamine amount in Lighvan cheese using HPLC method. Moreover, the chemical composition of EO was assessed using GC/MS. Cheese samples containing 0, 125 and 250 ppm of Rosemary EO were prepared. Afterward, lactobacillus count and histamine content of the samples were measured on days 0, 30, 90 and 150 of the ripening period. Alfa pinene (11.1%), Camphor (10.2%) and Limonene (5.9%) were detected as three main components of the rosemary EO. During the ripening period lactobacillus count in all EO-treated groups (125 and 250 ppm) was found significantly (p<0.05) lower than the control group. Moreover, histamine contents in the control group, and 125 and 200 ppm containing rosemary EO cheese samples were determined as 190, 170 and 51.04 mg/kg, respectively. It could be concluded that the addition of 125 and 250 ppm of rosemary EO could act as a natural preservative in traditional Lighvan cheese.

کلیدواژه‌ها [English]

  • Rosemary essence
  • Histamine
  • Lactobacilli
  • Traditional cheese
  • Ripening period
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