سنجش مولکولی تقلب گوشت مرغ در همبرگر‌ممتاز به روش Simplex PCR و Duplex PCR

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی‌ارشد صنایع‌غذایی، واحد علوم و تحقیقات، دانشگاه‌ آزاد اسلامی، تهران، ایران

2 دانشیار گروه علوم و صنایع‌غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 دانشیار گروه زیست‌شناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند، ایران

4 دانشیار گروه میکروبیولوژی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

گوشت یک منبع پروتئین عالی و مغذی محسوب ‌می­شود اما به­علت­ قیمت بالای این پروتئین­ حیوانی و دست‌یابی به سود اقتصادی بیشتر توسط برخی تولیدکنندگان، اغلب در فرآورده­های گوشتی، تقلباتی نظیر استفاده از گونه­های گوشتی با ارزش تجاری کمتر، مورد ‌توجه است. در ‌‌‌‌این ‌تحقیق سعی ­شده تا پروتئین ارزان ‌قیمتی نظیر گوشت‌ مرغ به‌روش مولکولی شناسایی­ گردد. از 10 نوع همبرگر ممتاز صنعتی با درج عنوان تهیه شده از گوشت گاو و نمونه‌های شاهد، شامل گوشت خام گاو و مرغ،استخراج  DNAانجام ‌گرفت و سپس واکنش‌های Simplex PCR و Duplex PCR بر روی DNA‌های حصول‌ یافته با استفاده از پرایمرهای ‌اختصاصی ویژه ژن سیتوکروم b گاو و s rRNA12 مرغ انجام ­‌شد. ژن‌های‌ ویژه‌ سیتوکروم ‌b‌ گاو و s‌rRNA 12 مرغ به‌­ترتیب قطعات bp 274 و bp 183 را طی واکنش‌های Simplex-PCR و Duplex-PCR در تمام نمونه­های همبرگر ممتاز تکثیر ‌کردند. تکثیر قطعه bp183 ویژه ژن مرغ طی واکنش‌های Simplex-PCR و Duplex-PCR، حاکی از جایگزینی تقلب مرغ با بخشی از گوشت گاو که ماده اصلی تمامی همبرگرهای ممتاز مورد آزمون است، می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Molecular assay of chicken meat fraud in premium burgers by Simplex and Duplex PCR

نویسندگان [English]

  • M. Hashemzadegan 1
  • E. Hosseini 2
  • F. Tafvizi 3
  • M. Bayat 4
1 M.Sc. Graduate in Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Associated Professor of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 Associated Professor of Biology, Parand Branch, Islamic Azad University, Parand, Iran
4 Associated Professor of Microbiology, Sciences and Researches Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Meat is considered an excellent and nutritious source of protein. However, due to the high cost of this animal protein and economic profit access of some producers, frauds like usage meat species of commercially lower value are noticeable. The aim of this study is to identify low-cost protein frauds such as chicken meat by the molecular method. DNA was extracted from 10 kinds of industrial premium beef burgers, and control samples involved raw beef and chicken meat. Then, simplex-PCR and duplex-PCR were run on the extracted DNA using specific primers of cytochrome b gene in beef and 12s rRNA gene in chicken. Specific genes of beef cytochrome b and chicken 12s rRNA amplified 274 bp and 183 bp fragments respectively in all premium burger samples in simplex-PCR and duplex-PCR. Amplification of fragments of 183 bp by simplex and duplex-PCR suggests adulteration of premium hamburgers by partial replacement of beef with chicken.

کلیدواژه‌ها [English]

  • Chicken Meat
  • Premium hamburger
  • Simplex-PCR
  • Duplex-PCR

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