جداسازی و شناسایی سویه‌های لاکتوکوکوس لاکتیس تولید کننده نایسین از پنیر لیقوان

نوع مقاله: مقاله پژوهشی

نویسنده

1. گروه بهداشت مواد غذایی و آبزیان، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران 2. مرکز تحقیقات بیوتکنولوژی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

چکیده

در این مطالعه سویه­هایی از باکتری­های اسید لاکتیک با قابلیت تولید باکتریوسین از نمونه­های پنیر لیقوان جداسازی شدند. در ابتدا از 16 جدایه لاکتوکوکوس لاکتیس شناسایی شده، چهار جدایه از نظر فنوتیپی به‌عنوان تولید کننده باکتریوسین تعیین شدند. جدایه­ها با روش لکه‌گذاری روی آگار بر رشد باکتری­های گرم مثبت بیماریزای غذایی شامل استافیلوکوکوس اورئوس، باسیلوس سروئوس، کلستریدیوم پرفرینجنس و لیستریا مونوسیتوجنز اثر بازدارندگی نشان دادند. در جدایه­های انتخاب شده واکنش زنجیره­ای پلیمراز برای ژن کد کننده نایسین مثبت بود. سویه­ها مواد ضدباکتریایی مقاوم به آنزیم آلفا آمیلاز اما حساس به تریپسین و پروتئیناز K تولید کردند. مقاومت حرارتی با تحمل 100 درجه سلسیوس به‌مدت 30 دقیقه مشخص شد. فعالیت ضدباکتریایی وابسته به pH بود و در pH 3 و 6 مشاهده شد اما در pH 9 تا حد زیادی کاهش یافت. پس از تیمار با SDS، Tween 20، Tween 80، Triton X-100، اوره و EDTA هیچ کاهشی در فعالیت ضدباکتریایی مشاهده نشد. طبق نتایج، این جدایه­ها قابلیت استفاده در مواد غذایی به‌عنوان نگه‌دارنده بیولوژیکی را دارند.

کلیدواژه‌ها


عنوان مقاله [English]

Isolation and identification of nisin producer strains of Lactococcus lactis from Lighvan cheese

نویسنده [English]

  • Kh. mohammadi
1. Department of Food Hygiene and Aquatics, College of Veterinary Medicine , Tabriz Branch, Islamic Azad University, Tabriz, Iran 2.Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran
چکیده [English]

In this study, strains of lactic acid bacteria capable of bacteriocin production were isolated from Lighvan cheese samples obtained from East Azerbaijan, Iran. Among 16 isolates initially identified as Lactococcus lactis, four isolates were phenotipically determined as bacteriocinogenic. The isolates showed inhibitory effect on the growth of Gram-positive food borne pathogens including Staphylococcus aureus, Bacillus cereus, Clostridium perfringens and Listeria monocytogenes by the agar spot test. Selected isolates were PCR-positive for nisin-encoding gene. The strains produced antibacterial substances resistant to α-amylase but sensitive to trypsin and proteinase K. Thermo-stability was indicated after treatment at 100 °C for 30 minutes. The antibacterial activity was pH dependent and occurred in pH 3 and 6, but at pH 9 the antibacterial activity was mainly restricted. No loss in antibacterial activity was recorded after treatment with SDS, Tween20, Tween 80, Triton X-100, urea and EDTA. The results suggest the potential application of these isolates as biopreservatives in food products.

کلیدواژه‌ها [English]

  • Lactococcus lactis
  • bacteriocin
  • Nisin
  • biopreservative
  • Lighvan cheese
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