مطالعه میزان آلودگی، مقاومت آنتی‌بیوتیکی، تولید بیوفیلم و وجود ژن TSST-1 در جدایه‌های استافیلوکوکوس اورئوس

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش‌آموخته کارشناسی ارشد علوم و صنایع غذایی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

2 استادیار گروه علوم و صنایع غذایی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران مرکز تحقیقات بیوتکنولوژی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

چکیده

هدف این مطالعه بررسی میزان آلودگی شیر خام و فرآورده‌های لبنی سنتی استان آذربایجان‌غربی با استافیلوکوکوس اورئوس و ارزیابی وجود ژن مولد زهرابه TSST-1، الگوی مقاومت آنتی‌بیوتیکی و قابلیت تولید بیوفیلم در جدایه‌های این باکتریبود. برای این منظور 80 نمونه شیر خام و فرآورده‌های آن (شامل پنیر، خامه و کشک سنتی) به‌همراه 20 نمونه سواب بینی تولیدکنند‌گان این محصولات با روش تصادفی طبقه‌ای جمع‌آوری شد. نمونه‌ها با استفاده از روش‌های متداول کشت، جداسازی و شناسایی گردیدند و سپس وجود ژن TSST-1 با استفاده از PCR ارزیابی شد. بر اساس نتایج مطالعه، 35 نمونه آلوده به استافیلوکوکوس اورئوس بودند که از این بین ژن TSST-1 در 1 نمونه پنیر و 2 نمونه سواب بینی ردیابی گردید. یافته‌های مقاومت آنتی‌بیوتیکی نشان داد اکثر جدایه‌های استافیلوکوکوس اورئوس نسبت به آنتی‌بیوتیک‌های پنی‌سیلین، ونکومایسین و متی‌سیلین مقاوم و نسبت به کوتریموکسازول،‌ جنتامایسین، ریفامپین، اگزاسیلین و سفولوتین حساس بودند. هم‌چنین تولید بیوفیلم در 85/2، 15/17 و 80 درصد جدایه‌ها به‌ترتیب زیاد، متوسط و کم برآورد گردید. فراوانی بالای آلودگی به استافیلوکوکوس اورئوس و وجود سویه‌های دارای ژن حدت TSST-1 در بین آن‌ها، هم‌چنین مقاومت بالا به آنتی‌بیوتیک مختلف و قابلیت تولید بیوفیلم، بیانگر مخاطره بهداشتی در ارتباط با مصرف فرآورده‌های لبنی سنتی است.

کلیدواژه‌ها


عنوان مقاله [English]

Contamination rate, antibiotic susceptibility profile, biofilm formation and presence of TSST-1 gene in Staphylococcus aureus isolates

نویسندگان [English]

  • K. Ebrahimzadeh 1
  • S. Hanifian 2
1 M.Sc Graduate in Food Science and Technology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Assistant Professor of Department of Food Science and Technology, Tabriz Branch, Islamic Azad University, Tabriz, Iran Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran
چکیده [English]

This study aimed to investigate the prevalence of Staphylococcus aureus in raw milk and traditional dairy products of West-Azerbaijan Province and also to evaluate the presence of TSST-1 virulence gene, antibiotic resistance and biofilm formation of the isolates. Using stratified random method, a total of 80 raw milk and traditional dairy products (including traditional cheese, cream and curd) together with 20 nasal swab samples of the dairy products’ manufacturers were collected. S. aureus strains were isolated and identified by conventional culture methods. Afterwards, the isolates were subjected to PCR analysis to detect the presence of TSST-1 gene. According to the findings, 35% of the samples were contaminated by S. aureus. Moreover TSST-1 gene was recognized in 1 cheese and 2 swab samples. Antibiotic resistance profile revealed that most of S. aureus isolates were resistant towards vancomycin, penicillin, and methicillin and sensitive towards co-trimoxazole, gentamicin, rifampin, oxacillin, and cephalothin. Moreover, 2.85%, 17.15%, and 80% of the isolates were capable to form high, moderate and low amounts of biofilm. High occurrence of S. aureus in milk and dairy products which harbor TSST-1 virulence gene, and the strains that demonstrated resistant to several antibiotics and capable of biofilm formation, could be considered a health threat to the consumers of these products.
 

کلیدواژه‌ها [English]

  • Staphylococcus aureus
  • TSST-1 gene
  • Antibiotic resistance
  • Biofilm
  • traditional Dairy products
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