نانوکپسولاسیون عصاره چای سبز به‌روش Thin film layer و ویژگی آنتی‌اکسیدانی و ضد‌میکروبی آن

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی تخصصی گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران

2 دانشیار گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران

3 استادیار گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران

4 استاد گروه بهداشت و کنترل مواد غذایی، دانشکده دامپزشکی دانشگاه تهران، تهران، ایران

چکیده

استفاده­ از ترکیبات طبیعی از جمله عصاره چای سبز در آماده‌سازی مواد غذایی و صنایع داروسازی محدود می‌باشد.کپسولاسیون مواد در نانولیپوزوم­ها می­تواند به‌عنوان یک سیستم محافظتی از ترکیبات طبیعی در طی فرآوری و نگهداری آن‌ها مورد استفاده قرار گیرد. در این مطالعه خصوصیات فیزیکوشیمیایی نانولیپوزوم عصاره چای سبز و همچنین محتوای فنولی، فعالیت آنتی‌اکسیدانی و  ضدمیکروبی آن مورد بررسی قرار گرفت.  فعالیت آنتی‌اکسیدانی با روش DPPH و خاصیت ضدمیکروبی به روش چاهک بر علیه باکتری‌های باسیلوس سرئوس، سالمونلا تیفی موریوم138  فاژتایپ 2، اشریشیا کولای O157:H7 و لیستریا مونوسایتوژنز تعیین شد. میانگین قطر نانولیپوزوم‌ها حدود 9/1 ± 7/44 نانومتر و شاخص پلی‌دیسپرسیتی 014/0± 203/0بود. بازده محصورسازی نانولیپوزوم چای سبز تحت شرایط بهینه 97٪ به‌دست آمد. فعالیت ضدمیکروبی عصاره چای سبز به‌طور معنی‌داری پس از کپسوله کردن در نانولیپوزوم افزایش یافت (05/0P<). بیشترین فعالیت ضد­میکروبی نانولیپوزوم چای سبز مربوط به باکتری لیستریا مونوسیتوژنز با منطقه مهار رشد 2/16 میلی‌متر بود، درحالی‌که باکتری اشریشیا کولای با هاله عدمرشد 14 میلی‌متری مقاوم‌ترین باکتری شناسایی شد. علاوه‌بر این، فعالیت آنتی‌اکسیدانی عصاره آبی چای سبز پس از کپسولاسیون در نانولیپوزوم به‌طور معنی‌داری افزایش داشت (05/0P<). به‌طوری که میزان  IC50 آن به 78/1میکروگرم در میلی‌لیتر کاهش یافت. براساس یافته‌های این تحقیق می‌توان گفت که نانوکپسولاسیون به‌طور مؤثری تأثیرات مفید عصاره چای سبز از جمله خواص ضد‌میکروبی و فعالیت‌های آنتی‌اکسیدانی آن را افزایش می‌دهد. لذا جهت افزایش پایداری ترکیبات طبیعی در طی فرایندهای مختلف پیشنهاد می‌گردد.

کلیدواژه‌ها


عنوان مقاله [English]

Nanoencapsulation of green tea extract by thin film layer method and its properties

نویسندگان [English]

  • B. Noudoost 1
  • N. Noori 2
  • H. Gandomi 3
  • A. Akhondzadeh Basti 4
2 Associate Professor of Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Assistant Professor of Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
4 Professor of Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
چکیده [English]

The application of natural compounds including green tea extract (GTE) in food preparation and pharmaceutical industries is limited. Encapsulation in nanoliposomes could be used as a delivery system to protect these compounds during processing and storage. In this study physicochemical characterization, total phenol content and antibacterial and antioxidant activity of green tea extract encapsulated in nanoliposomes were evaluated. GTE was encapsulated in liposomes by thin film layer method and reached to nanoscale with sonication. The antioxidant activity of nanoliposomal GTE was estimated by DPPH assay. The antibacterial activity of nanoliposomal GTE against Bacillus cereus (ATCC11778), Salmonella typhimurium 138 phage type 2, Escherichia coli O157:H7 and Listeria monocytogenes (ATCC19118) was determined using well diffusion technique. The mean diameter of nanoliposomes was about 44.7±1.9 nm and had 0.203±0.014 polydispersity index. Entrapment efficiency of nanoliposomal GTE under the optimum conditions was 97%. Antibacterial activity of GTE was significantly increased after encapsulation in nanoliposomes. The strongest antibacterial activity of nanoliposomal GTE was seen against L. monocytogenes with an inhibition zone of 16.2 mm while E. coli was the most resistance strain with an inhibition zone of 14 mm. Furthermore, the antioxidant activity of GTE was significantly increased after nanoliposome encapsulation since the IC50 value of nanoliposomal GTE was decreased to 1.78 μg/ml. Nanoencapsulation effectively enhanced beneficial properties of GTE including antimicrobial and antioxidant activities.

کلیدواژه‌ها [English]

  • Green tea extract
  • Nanoliposome encapsulation
  • Antioxidant Activity
  • antibacterial activity
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